Parallel regulation of cyclic amp-dependent protein kinase and phosphoprotein phosphatase in rat thyroid
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چکیده
منابع مشابه
Role of phosphorylase kinase and cyclic AMP-dependent protein kinase in the regulation of phosphorylase phosphatase.
The enzymes of glycogen metabolism are regulated by interconversion between phosphorylated and dephosphorylated forms. In contrast with our well-defined knowledge of the phosphorylation processes of different enzymes and their role in glycogen breakdown and synthesis, the phosphatases catalysing the reverse processes are only now being understood. The regulation of dephosphorylation reactions i...
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The roles of cyclic AMP and cyclic AMP-dependent protein kinase in the contractile and metabolic responses to neural stimulation were studied in the bullfrog heart perfused in situ. The interaction of parasympathetic and sympathetic nerves in the regulation of myocardial cyclic AMP, cyclic AMP-dependent protein kinase, and phosphorylase also was studied. Stimulation of the left vagosympathetic ...
متن کاملCyclic AMP-induced expression of steroidogenic acute regulatory protein is dependent upon phosphoprotein phosphatase activities.
In addition to the well-documented role of protein kinases in the regulation of steroid production, phosphoprotein phosphatase (PP) activity is required for steroidogenesis. In the present study, we have used the mouse Y1 adrenocortical cell line to identify the site of action of PPs on steroid production by measuring the effects of PP inhibition on the expression of the steroidogenic acute reg...
متن کاملby cyclic AMP-dependent protein kinase
Myosin light-chain kinase was purifed from bovine carotid artery. Approx. 90% of myosin kinase was extracted in the supernatant fraction with buffer containing EDTA during myofibril preparation. The soluble fraction yielded two distinct peaks on DEAE-Sephacel chromatography. Peak I was eluted at a conductance of 11-12mmho and was completely dependent on Ca2+-calmodulin for its activity. Peak II...
متن کاملFluorometric assay for adenosine 3',5'-cyclic monophosphate-dependent protein kinase and phosphoprotein phosphatase activities.
A novel peptide substrate for adenosine 3',5'-cyclic monophosphate-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37), Leu-Arg-Arg-Trp-Ser-Leu-Gly, was synthesized. Phosphorylation of the peptide causes a 20% increase in the peptide fluorescence intensity at 358 nm. Values of Km and kcat for the phosphorylation reaction at pH 7.0 (25 degrees C), were determined to be 2.7 +/-...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80271-9